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To measure NMDA-induced inward current from GLP-1-receptor-positive neurons, we used magnesium-free aCSF (126 mM NaCl, 2.8 mM KCl, 2.5 mM CaCl 2 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 and 5 mM glucose) containing 10 μM CNQX and 100 μM picrotoxin
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Following this, a new collection of 3-pyridylacetamide analogs with an additional hydrogen-bond acceptor was designed, which could preferentially engage in bidentate interactions with Arg125
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