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Electron microscopy MIN6B1-SNAP-GLP-1R cells cultured on Thermanox coverslips (Agar Scientific) were labeled with 2 μM cleavable SNAP-Surface biotin probe in HEPES-bicarbonate buffer plus 3 mM glucose and 1% BSA, followed by incubation with 5 μg ml −1 NaN 3 -free Alexa Fluor 488 Streptavidin, 10 nm colloidal gold conjugate (Molecular Probes) and stimulation with 100 nM agonist in buffer plus 11 mM glucose
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