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The concentrated product was loaded onto a Superdex 200 increase 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5), 100 mM NaCl, 10 mM MgCl 2 , 100 μM TCEP, 1 μM GLP-1 or LY3502970 and/or 5 μM compound 2, 0.00075% LMNG, 0.00025% GDN and 0.0002% (w/v) CHS
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