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Briefly, 0.5 mg of labeling dye was dissolved in 50 µl DMSO and was slowly added to 1 ml of protein (∼10 mg/ml) in 0.1 M sodium bicarbonate buffer (pH 8.3)
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Furthermore, the effects of the various forms of CCK, namely, CCK-8, 33, and 58, differ depending on their site of action in the gastrointestinal tract 162,163,164
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